Journal: Journal of Applied Genetics

Article Title: Long-run real-time PCR analysis of repetitive nuclear elements as a novel tool for DNA damage quantification in single cells: an approach validated on mouse oocytes and fibroblasts

doi: 10.1007/s13353-023-00817-0

Figure Lengend Snippet: The L1-LORD-Q assay allows quantifying the number of DNA lesions in single oocytes treated with different doses of UV light and shows a level of damage comparable to the standard immunofluorescent method. A Exemplary control and UV-treated oocytes stained with antibody against γ-H2AX—a popular marker of DNA double strand breaks. B Level of DNA breaks in control and UV-treated oocytes assessed by immunofluorescent labeling of γ-H2AX foci (means ± SEM, in brackets: number of oocytes analyzed in each group). C Number of DNA lesions in control and UV-treated oocytes assessed by the modified LORD-Q assay (means ± SEM, in brackets: number of oocytes analyzed in each group)

Article Snippet: Then, the oocytes were incubated overnight at 4 °C with the primary anti-γ-H2AX antibody (Cell Signaling Technology, Danvers, MA, USA, 9718) diluted 1 : 100 in 10% FBS.

Techniques: Control, Staining, Marker, Labeling, Modification

Journal: Oncology reports

Article Title: Inhibitory effects of valproic acid in DNA double-strand break repair after irradiation in esophageal squamous carcinoma cells.

doi: 10.3892/or.2015.4089

Figure Lengend Snippet: Figure 1. Effects of VPA on radiation-induced DNA DSBs. ESCC cells KES, TE9 and TE11 were cultured with or without VPA (0.5 mM) for 24 h, then irradi- ated (IR, 6 Gy). H2AX phosphorylation (γH2AX) levels, as a sensitive marker of radiation-induced DSBs, were determined by western blotting at designated times (0/10/30 min/1/2/4/8 h). β-actin was used as a loading control in each blot. VPA, valproic acid; DSBs, DNA double-strand breaks; ESCC, esophageal squamous cell carcinoma.

Article Snippet: Rabbit anti-phospho-histone H2AX (Ser 139) (γH2AX) (cat. #9718) and anti-acetylatedlysine monoclonal antibodies (cat. #9814) were obtained from Cell Signaling Technology Co. Ltd. (Tokyo, Japan).

Techniques: Cell Culture, Phospho-proteomics, Marker, Western Blot, Control

Journal: Oncology reports

Article Title: Inhibitory effects of valproic acid in DNA double-strand break repair after irradiation in esophageal squamous carcinoma cells.

doi: 10.3892/or.2015.4089

Figure Lengend Snippet: Figure 2. Time course of the H2AX phosphorylation level changes after irradiation. γH2AX levels were detected by western blotting in KES, TE11 and TE9 cells cultured with (filled squares) or without (filled triangles) VPA, and were then irradiated. Values were quantified by measuring contrasting densities and calculated as the ratio of γH2AX to β-actin. VPA, valproic acid.

Article Snippet: Rabbit anti-phospho-histone H2AX (Ser 139) (γH2AX) (cat. #9718) and anti-acetylatedlysine monoclonal antibodies (cat. #9814) were obtained from Cell Signaling Technology Co. Ltd. (Tokyo, Japan).

Techniques: Phospho-proteomics, Irradiation, Western Blot, Cell Culture

Journal: Oncology reports

Article Title: Inhibitory effects of valproic acid in DNA double-strand break repair after irradiation in esophageal squamous carcinoma cells.

doi: 10.3892/or.2015.4089

Figure Lengend Snippet: Figure 3. Changes over time in the H2AX phosphorylation foci formation after irradiation, as assessed by immunofluorescent cytochemistry. Micrographs of γH2AX foci (red) in nuclei (blue) of KES and TE9 cells pre-treated with or without VPA (0.5 mM) for 24 h were obtained (0 h/4 h/8 h) after irradiation (IR). VPA was shown to prolong γH2AX foci formation after irradiation. Scale bar, 10 µm. VPA, valproic acid.

Article Snippet: Rabbit anti-phospho-histone H2AX (Ser 139) (γH2AX) (cat. #9718) and anti-acetylatedlysine monoclonal antibodies (cat. #9814) were obtained from Cell Signaling Technology Co. Ltd. (Tokyo, Japan).

Techniques: Phospho-proteomics, Irradiation